Affymetrix GeneChip expression arrays are available for over 30 different eukaryotic
and prokaryotic species, and new arrays are being added on a regular basis.
Currently, the Core Laboratory provides various arrays (Tiling, Exon and Gene array)
and Genotyping array services, including genotyping, microRNA, gene expression,
transcript mapping and cytogenetics study. We also have the capacity to expand
to run new arrays available in Affymetrix. For details of the technology, please visit
www.affymetrix.com.
Applied Biosystems 7900 HT Fast Real-time PCR System and TaqMan Low
Density Array
The Applied Biosystems 7900 HT Fast Real-time PCR system is a powerful research
tool for high throughput DNA/RNA quantification, allelic discrimination and plus/
minus assay.
Following Software are installed in Applied Biosystems 7900HT workstation:
1. SDS 2.3
2. RQ Manager 1.2
3. Primer Express v. 3
Standard 384-well block is currently in use. Registered users are welcomed to use
the machine for free with advance booking.
Illumina iScan System
Beadchip based genetic analysis assays for genotyping, CNV analysis, DNA
methylation and gene expression profiling.
Fluidigm BioMark System
The Fluidigm BioMark System is a high-throughput real-time PCR system. It
enables real-time quantitative PCR (qPCR), SNP genotyping, gene expression
profiling and digital PCR assays using the Integrated Fluidic Circuit (IFC)
technology, a high-throughput and low volume fluidics chip. The BioMark System
streamlines workflow for applications demanding sensitivity and dynamic range.
The system acquires data for each reaction chamber on the IFC simultaneously
and can operate in either end-point or real-time detection mode.
For details of the System, please visit Fluidigm’s website.
The Core Laboratory is offering the BioMark Platform on a self-service and
first-come, first-served basis (located in Room 505, Li Ka Shing Medical
Sciences Building, Prince of Wales Hospital). Users should bring their own
reagents and consumables and prepare the array chips by themselves. The usage
fee will be charged on cost-recovery basis only.
