c. Quantification & Quality Assessment
Agilent 2100 Bioanalyzer
The Agilent 2100 bioanalyzer is a microfluidics-based platform which is replacing
the labour-intensive formaldehyde gel electrophoresis. The bioanalyzer platform is
available for the analysis of DNA, RNA, protein and cells. It only takes 1 hour for the
entire assay with automated delivering high quality digital data output. The Core
Laboratory currently provides RNA quality check service using the Agilent RNA 6000
Pico Total RNA kit (Cat# 5067-1513) which are designed for the analysis of total
RNA (eukaryotic and prokaryotic) and mRNA samples. The ratio of RNA fragments
(either 18S and 28S for eukaryotic RNA or 16S and 23S for prokaryotic RNA) is
calculated and displayed. Moreover, the area under the entire RNA electropherogram
gives the concentration of the RNA.
The NanoDrop® ND-2000
The NanoDrop® ND-2000 is a full-spectrum (220-750nm) spectrophotometer. The
newly developed sample retention technology enables the measurement of as small
as 1ul sample droplet. Simply wiping both the upper and lower pedestals prevents
sample carryover in successive measurements for samples varying in concentration
by as much as 1000 fold. Registered users are welcome to use for free.