Research
Cell Biology Core Facilities
Class II Type A2 Biosafety Cabinet
Class II Type A2 Biosafety Cabinet
Our Cell Culture Facilities feature three dedicated cell culture rooms located on different floors, providing enhanced spatial separation and workflow flexibility. Each room is equipped with two certified Class II Type A2 Biosafety Cabinets, giving a total of six BSCs across the facility.

These biosafety cabinets support the culture of both animal- and human-origin cell lines under stringent aseptic conditions. Benefiting from a recent expansion of our facilities, this multi-room setup significantly increases our cell culture capacity, reduces the risk of cross-contamination between projects, and allows multiple research teams to work simultaneously with greater convenience and efficiency.
#Cell Culture Facilities
Molecular Biology Core Facilities
Amaxa Nucleofector II
Amaxa Nucleofector II
The Amaxa Nucleofector II is a transfection device that utilises electrical pulses to effectively transfect nucleic acids and small molecules into a wide range of cell types, including primary cells and difficult-to-transfect cell lines.

Equipped with pre-optimised transfection buffers as well as tailor-made programs for more than 100 cell types, the Nucleofector II is also armed with a closed system design to minimise contamination to facilitate highly efficient transfections with a high cellular viability rate.

Its scalable sample capacities are also favoured by scientists for transfections that are crucial for advanced cell biology research, gene expression studies, cell engineering and much more.
#Electroporation Transfection
Molecular Biology Core Facilities
Bio-Rad QX200 Droplet Digital PCR System
Bio-Rad QX200 Droplet Digital PCR System
The Bio-Rad QX200 Droplet Digital PCR (ddPCR) system offers precise, absolute nucleic acid quantification without the need for any standard curves.

By partitioning the samples into thousands of nanolitre-sized oil droplets, this technology enables the reliable detection of rare genomic mutations, copy number variations, and precise quantification of nucleic acids with high sensitivity and reproducibility.

Applications include cancer research for detecting low-frequency mutations, viral load quantification in infectious diseases, and accurate gene expression analyses et cetera.
#Gene Expression Profiling, Analysis and Genotyping
Molecular Biology Core Facilities
Life Technologies QuantStudio 12K Flex Real-Time PCR System
Life Technologies QuantStudio 12K Flex Real-Time PCR System
The QuantStudio 12K Flex Real-Time PCR System supports high-throughput quantitative PCR applications, accommodating a 96-well or a 384-well plate per run. It features an interchangeable heat block format for research flexibility, including the TLDA (TaqMan Low Density Array) block.

The system is equipped with advanced optical components that enable precise and sensitive detection across multiple fluorescence channels, supporting gene expression, genotyping, and copy number variation analyses using various fluorescent dyes (e.g., TaqMan and SYBR Green). It also offers automated data analysis and comprehensive software for experiment setup, data interpretation, and report generation.
#Gene Expression Profiling, Analysis and Genotyping
Cell Biology Core Facilities
BD FACSAria Fusion Flow Cytometry Cell Sorter
BD FACSAria Fusion Flow Cytometry Cell Sorter
The BD FACSAria Fusion Flow Cytometry Cell Sorter provides a precise and efficient separation of complex cell populations. It enables the simultaneous detection and sorting of cells, up to 18 parameters, ensuring an in-depth phenotypic analysis with gentle and targeted sorting that minimizes impact on cell viability.

The certified biosafety cabinet features a biocontainment design that ensures users’ safe handling of hazardous samples.

The flow cytometer is configured with 640 nm, 561 nm, 488 nm, 405 nm, and 375 nm excitation lasers, and supports the detection of PE, PE-Texas Red, PE-Cy5, PE-Cy5.5, PE-Cy7, DAPI, AmCyan, BV605, APC, Alexa Fluor 700, APC-Cy7, FITC, and PerCP-Cy5.5 fluorochromes.

This system is compatible with various sample types of collection devices, including single-cell deposition, benefiting researchers in field of immunology, stem cell biology, and cancer research in their downstream applications.
#Cell Analysis and Sorting
Molecular Biology Core Facilities
Illumina iScan Microarray System
Illumina iScan Microarray System
The iScan system provides accurate data with a high resolution for genome-wide association studies (GWAS), gene expression profiling, and methylation analyses.

Its automation and scalability make it the suitable device for a diverse range of research applications, including the understanding of complex genetic diseases, novel biomarker discovery, and pharmacogenomics research et cetera.
#Gene Expression Profiling, Analysis and Genotyping
Molecular Biology Core Facilities
Bio-Rad ChemiDoc MP Imaging System
Bio-Rad ChemiDoc MP Imaging System
The ChemiDoc MP Imaging System combines advanced gel/blot imaging capabilities with versatile detection methods i.e. fluorescence, chemiluminescence, and colorimetric detections. It supports a variety of fluorescent reporters in a multiplexed manner, providing the users with a wide breadth of versatility.

Its auto-focusing, exposure fine tuning and simplified sample previewing, and the support of stain-free protein workflows are all beloved merits of this instrument. The stain-free approach saves time by eliminating lengthy staining and destaining steps. It provides more accurate and efficient protein quantitation and preserves proteins for further analysis like mass spectrometry or Western blotting. In addition, the automatic recognition feature of the Smart Trays ensures hassle-free automatic selection of the correct protocols based on the imaging tray in use.

This system is mainly used in protein/nucleic acid gel documentation, western blotting, and protein-ligand interaction analysis et cetera.
#Multi-purpose Imaging System
MOLECULAR BIOLOGY CORE FACILITIES
Thermo Fisher Scientific Nanodrop ND-1000 & ND-2000 Spectrophotometers
Thermo Fisher Scientific Nanodrop ND-1000 & ND-2000 Spectrophotometers
The Nanodrop Spectrophotometer is a compact instrument designed for the rapid and accurate quantification of nucleic acids, proteins, and other biomolecules. It utilises a patented sample retention system that comes with a minimal sample volume requirement (as low as 0.5 μL).

The spectrophotometer covers a wide range of UV-Vis wavelengths, thus allowing the measurement of sample purity and concentration without even the need for measurement cuvettes.

It features intuitive software for data analysis and includes pre-configured methods for the most common assays.
#Sample Quality Assessment
Core Facilities
Core Facilities at the Li Ka Shing Institute of Health Sciences (LiHS)
The Core Facilities at the Institute serve as a centralised resource to support the research needs of scientists across the University. They provide a wide range of services, including:
  • Molecular Biology Core Facilities
  • Cell Biology Core Facilities

The Molecular Biology Core Facilities offer a comprehensive platform for molecular research, enabling researchers to perform a variety of fundamental and crucial routine experimental procedures. These include, but are not limited to, Sample Quality ControlTransfectionMolecular Analyses and others.

The Cell Biology Core Facilities provide a series of comprehensive cellular research platforms for cell-biology related work, such as Cell Selection and Cell Culturing.

Core Facilities

If you wish to reserve core laboratory facilities at the Li Ka Shing Medical Sciences Building, Prince of Wales Hospital, please submit your booking request via email to lihscore@cuhk.edu.hk

 

For new registration for core laboratory facilities, please complete the Online Form here for further arrangements. For other laboratory related inquiries, please contact our Core Laboratory:

Miss Carol SZETO
(852) 3763 6004
Room 804, 8/F Li Ka Shing Medical Sciences Building, Prince of Wales Hospital, Shatin, N.T., Hong Kong